Product Overview:

DIC: 20x (0.17 mm).
DIC: 40x (0.17 mm).
DIC: 40x (0.17 mm).
(Not configured for long working distance)
Magnification Changer: 1x, 1.6x, 2.5x.
Full mechanical stage, right hand.
Universal stage plate adapter – slides and petri dishes.
Top photography port.
HBO 100 Illuminator.

Main Features:

Large XY mechanical stage with stage plate to fit petri dishes or standard glass slides. Not the cheap version that simply slides the adapter plate over a fixed stage. Stage is extra large size.
Right Hand XY stage slider movement.
Tilt back upper arm allows easy access to specimen vessels. Simply tilt the arm out of the way to access your petri dish.
Five-place objective nosepiece, with all having DIC openings.
Eyepieces: E-Pl 10x/20 pair with diopters on both.
Voltage: The frame has a volt switch 115v/230v. The HBO power supply is autovolt.

Photography System:

Photography port on top of head. It has a light path selector with ability to take 100% light to the eyepieces or 100% light to the top photo port.
Ability to shut out extraneous light from the eyepieces that may enter the camera optics.
C-mount attachment for camera is included.
DOES NOT INCLUDE CAMERA. Camera is shown in images to demonstrate that one can be connected.

Differential Interference Contrast – DIC:

This is a fully loaded DIC configuration.
DIC Magnifications:
20x (0.17 mm coverslip thickness).
40x (0.17 mm coverslip thickness).
100x (0.17 mm coverslip thickness).
We tested the DIC on all magnifications and obtained excellent textbook quality relief type images for each. Everything works as it was designed to.
DIC is a microscopy technique that gives relief-like images, especially with thick specimens. The 3D effect enhances visualizing structures in thick specimens better than phase. Thick specimens normally are not imaged well in phase contrast. This microscope is capable of imaging in relief or pseudo darkfield, depending on how you adjust it.
With DIC, you are limited to use of glass holders. If you need to use plastic holders, then we can provide a unit with PlasDIC instead.

Fluorescent Illumination:

Upgraded HBO 100 Lamp Housing and External Power Supply with included 100W Mercury Bulb.
Luminous Field Iris Diaphragm on epifluorescence light path.
Shutter for epifluorescence illumination.
IR heatshield (epi-illumination light path) is properly installed to protect internal optics.
Includes IR filter on the slider for additional protection, specifically to reduce photo bleaching on the fluorescent specimen.
Proper optical alignment was achieved in the epi illumination light path. These frames often have internal problems and are not able to achieve alignment, ultimately affecting imaging quality.

Fluorescence Dichroic Filters:

Filter Slider with three settings. DAPI, FITC, TRITC.
These dichroic filters are in perfect condition without any visible where. They were all tested and produced excellent expected results.
DAPI (UV): Ex. 365, Bs. 395, Em. 420 longpass.
FITC (Blue): Ex. 450-490, Bs. 510, Em. 515-565 bandpass.
TRITC (Green): Ex. 546, Bs. 580. Em. 590 longpass.
My Note: There is no open space for Brightfield and DIC Observation. So to view those, you need to pull out the slider tray.

Transmitted Illumination: Brightfield – Phase Contrast

Rotary Turret style light Condenser with phase contrast and DIC settings.
High Numerical Aperature. 0.55 N.A. Better for obtaining higher resolution images, especially with the higher power magnifications.
4-Place Condenser has Settings for: Ph1, Ph2 and DIC (2).
While no phase objectives have been included, this microscope is completely phase compatible with the phase annuli already installed in the condenser. To add phase contrast microscopy, you only need to include some phase objectives.
Condenser Iris diaphragm.
Koehler Field Diaphragm. Proper Koehler illumination and centering were obtained.
12V-100W Halogen with two spare bulbs.
Transformer is built into the main frame and not an external unit.
Two empty swing-out filter holders for optional filters.

Objective Lenses:

10x. Achroplan 10x/0.25, 8/-. This gave excellent images in brightfield but is not recommended for fluorescence.
20x. Plan-Neofluar 20x / 0.50, 8/0.17. Spring. This gave great imaging in BF, DIC, and fluorescence.
40x. Plan-Neofluar 40x / 0.75. 8/0.17 mm. Spring. This gave great imaging in BF, DIC, and fluorescence.
100x. Plan-Neofluar 100x / 1.30, Oil, 8/0.17. This gave great imaging in BF, DIC, and fluorescence.

Optovar Magnification Changer:

Optovar lens Magnifications: 1x, 1.6x, and 2.5x.
This frame has a built-in magnification changing system. It contains optovar lenses that change the final viewing magnification. This is very useful for increasing magnification when you don’t want to change the objective.
Comments:
This microscope is in great condition.
It also has all the tools included for proper assembly and disassembly.
Includes all needed power cords.

Optional Accessories:

We have a wide variety of optional scientific cameras, both monochrome and color, with and without Peltier cooling.
Other objectives can be configured.

Good Value:

When comparing prices, keep in mine the extras that we are providing.
We are including a one year warranty, money back guarantee, and free shipping that are value added additions not given by most resellers.
We have carefully refurbished this item and completely tested all functionality. Everything is working well and as designed.

User Manual and Instructions:

Upon purchase, we will email links to the user manuals / instructions.