ITEM CODE: SKU-018
Shipping Cost: Free Worldwide Shipping
Main Features:
- Tilt back upper light carrier. Ability to tilt out of the way to access large culture vessels or optional micromanipulation equipment.
- Stage has XY slider with a universal mounting plate capable of handling petri dishes or a typical 1×3” glass slide.
- Binocular Viewing Head.
- Eyepiece pair is W-Pl 10x/23 guaranteed for usage with eyeglasses or without. The 23mm is a great field of view. Both eyepieces have focusing diopters.
- Shutter with ability to block light from entering eyepieces when taking photos. This prevents inadvertent ambient light exposure on camera.
- Six Place Objective Nosepiece. With DIC slots in three positions.
- Rear lifting handle to make it easier to move this large and heavy duty instrument.
- Large physical size gives excellent thermal and mechanical stability.
- All required power cords.
- Side camera photography port. This has ability to take 100 percent light to eyepieces or 100 percent to camera port.
- C-Mount for attaching a camera.
- DOES NOT INCLUDE CAMERA OR LAPTOP. Camera is shown in images to demonstrate that one can be connected.
Transmitted Light – Brightfield and Phase Contrast Microscopy:
- HAL 100 lamp housing. With 100W halogen bulb.
- Includes diffusion disk for proper illumination of field of view from the halogen filament.
- Rotary Light Condenser.
- Numerical Aperture 0.35 N.A. 70mm Working Distance.
- Settings for: Ph0, Ph1, Ph2, DIC, DIC, H. The “H” is brightfield.
- Has two Differential Interference Contrast settings but no prisms installed.
- Capablity for Upgrade to DIC. Has Empty DIC slots, ready to accept prisms.
- Condenser centering screws.
- Variable light intensity rotary knob.
EpiFluorescence Reflected Light System:
- HBO 100. Lamp housing and power supply. With 100W Mercury bulb.
- Six positions for dichroic cubes (reflector modules) in the reflector turret.
- Filter holder with three empty slots for optional filters. Use this as a shutter when pushed all the way to one side.
- Aperature Diaphragm and Field Diaphragm sliders.
Objectives:
- EC Plan-NeoFluar 2.5x/0.075. Infinity/0.17. Part#440310-9903. Not Phase. (not optimal for petri dish but will work).
- A-Plan 5x/0.12 Infinity/-. Ph0. Part#1018-589. Phase.
- LD Plan-NeoFluar 20x / 0.4 Korr. Infinity/0-1.5. Correction collar with adjustment for 0 to 1.5mm thick cell culture vessel bottom. Not Phase. Part#421350-9970
- LD Plan-NeoFluar 40x / 0.6 Ph2, Korr. Infinity/0-1.5. Correction collar with adjustment for 0 to 1.5mm thick cell culture vessel bottom. Phase. Part#421361-9970
Filters – Dichroic Cubes:
- Does not include any filter cubes.
- This was tested with a brand new FITC cube and it performed well. It is not included.
Zeiss Tool Kit:
- Includes the original Zeiss supplied toolkit. Made in Germany. These include the needed screwdrivers and spanner wrenches, etc, as noted in the user manual.
Comments:
- This microscope is in great condition.
Research Grade Applications:
- This optical instrument is used for observing intracellular processes in living cell cultures, motility and growth, cell to cell interaction, and drug detection. With optional hardware equipment and software, it can also do micromanipulation and microinjection, IVF in-vitro fertilization, patch-clamp technique, toxicity examinations, time lapse examinations, and ion measurements. It can also be useful for z-sectioning, Fura (Ca measurement), deconvolution, GFP, and single molecule detection. This is a high grade research model and is capable of being adapted to all of the above, given the right optional equipment.
Quality:
- This is a high quality Zeiss (Made in Germany) optical instrument.
- We also sell Chinese made fluorescence microscopes but the quality is substantially lower.
- We highly discourage buying the Chinese microscopes due to the problems that incur after the sale.
Testing Results:
- This microscope was fully tested. Results are as follows:
- All functions were found to be in proper working order.
- Brightfield – Koehler illumination was set properly, with all adjustments and centering being properly achieved.
- This is only a minor inconvenience, but when raising and lowering the condenser (done very seldom, only to set Koehler illumination), you need to use two hands on both knobs, otherwise, one knob tends to slip slightly.
- Phase Contrast – All objectives performed well under phase. Images achieved were good.
- Incident Reflected Fluorescence – HBO Bulb was properly centered obtaining bright, clear, and uniform illumination distribution across the complete field of view.
- Fluorescing Achieved –
- Tested with a FITC cube, not included. It was fine.
- Inspection of the lenses inside the reflected illumination system (for fluorescence illumination) showed a very clean and clear optical path. These usually get a permanent haze etched on the glass after some usage. It appears this unit has had little to no usage of reflected light.
Optional Accessories:
- We have a wide variety of optional scientific cameras, both monochrome and color, with and without Peltier cooling.
- Other stage mounting plates are available for use with other sizes of petri dishes and cell culture vessels.
- DIC differential interference contrast is possible on this frame. Contact us for details about how to add this technique.
- Other objectives can be configured.
- We can provide brand new replacement 100W HBO Mercury Bulbs and Halogen bulbs.
- Brand new Chroma hard coated filter cubes with lifetime warranty may be added. Cost is USD $750/set for the dichroic filter (emission filter, dichroic mirror, barrier filter) and includes the blank cube.
User Manual and Instructions:
Click this link: Zeiss Axio Observer Manual.pdf
Click this link: Zeiss Axio Observer Brochure.pdf
TERMS AND CONDITIONS:
One Year Parts and Labor Warranty:
We warrant this Microscope to be free from defects due to normal usage in normal operating conditions for a period of one year. Camera equipment (if included) is given a three month warranty. Objective lenses are excluded from warranty.
Additional warranty on microscope may be purchased if desired.
We will repair or replace equipment at no charge, during the one year warranty period. Both parts and labor are included. Buyer is responsible for shipping costs BOTH ways, paying for shipping cost to our designated repair facility and shipping cost back to buyer. Bulbs are considered user replaceable items and not covered.
Warranty Excludes Objective Lenses:
Our warranty excludes the objective lenses. There are several reasons for this. First of all, if the objective is good, it will continue good performance unless there is some user or environmental abuse. Abuse can be from oil getting on a non-oil immersion lens, contact with a solution that degrades the cement around the glass, contact with other chemicals that may stain, etch or pit the glass, scratching the lens by incorrect cleaning methods such as cloths not of lens cleaning quality, damaging the lens by scraping it on slides or metal slide holders on the stage, dropping on a hard surface, subjecting the objective to extreme humidity causing internal mold or mildew, etc.
In addition, objectives can’t feasibly be repaired. If an objective is damaged so that the optical quality is significantly diminished, it is suggested to throw it away. One solution is to send it back to the factory and incur a repair fee equal to about half the price of a new replacement. That is usually cost prohibitive. We professionally clean the front and back lens, and the outside barrel of the objective. But we do not open them up. That should only be done by the factory under strict conditions and with optical laser alignment. Generally, when someone other than the factory messes with a lens, the performance may still be acceptable, but likely will be noticeably lower quality when compared side by side with a new lens. So since defective objectives are generally thrown away, and replaced with another one, there is no need to warranty them. The user can easily find a matching objective and replace it on their own.
Objective Lenses – Condition:
All objectives have been professionally cleaned.
All are guaranteed to be free from optical defects. If any defect is found, it will be clearly noted in the ad.
Our inspection of the objective lenses includes checking for the following:
lens cracking, pitting, lens cement not intact, internal oil damage or oil seepage, glass fogging, glass hazing, stains on optical glass, mold or mildew stains, and delamination (separation of internal lenses).
Condition of Equipment:
This reconditioned microscope is complete and in excellent working order and has been completely checked out and serviced.
Technical Sales and Support:
Most offerings of used microscopes are by simple flippers of equipment who have obtained defective and rejected microscopes from labs discarding the equipment. They resell exactly what they got, as they got it, and without any technical knowledge of it. You can rest assured when purchasing from us that we are professional dealers who are well trained to service and provide technical support of the microscopy equipment. We test, repair and refurbish all equipment to excellent working conditions. We are well trained and able to give you technical advice including pre-sale and post-sale support.
Microscope Training – Setup Support – Software Assistance:
Concerned about how to set up and operate this equipment? Need training? Need help with software for the camera? We can help. Contact us for more details.
Free Application Evaluation and Recommendation:
We strongly encourage all potential buyers to first contact us with regards to their specific application. In most situations, we need to customize certain features of the microscope in order to best fit the end user’s technical requirements. We want to ensure you have the correct and optimal configuration for your application.
Fluorescence Filter Sets – Customized:
We are dealers of both Chroma and Omega brands (Made in USA) of dichroic filters. Please contact us regarding the specific fluorophores you are using so we can be sure the filter sets provided are a proper match. Incorrect matching will give you poor quality or no visible fluorescing.
Free Worldwide Shipping:
Equipment may ship from Outside USA. We provide free worldwide shipping. The method of shipping may vary, depending on destination country, but may be via commercial air cargo. Not all countries will have door to door service. Contact us for further details so we can discuss shipping arrangements.
Customs Duty and Tax:
Any and all customs brokerage, customs entry, VAT and duty/tax are the responsibility of the buyer. Buyers in USA can contact us in advance to see if there are any entry fees.
Voltage Requirement:
Our equipment may show 110V and/or 220V external power supplies. Some power supplies are not Volt selectable. Depending on your country of final destination, and the Voltage supply, we may need to change the power supply shown in the ad to one that matches your power requirements. Contact us first with your Voltage requirement so we can be certain we have the required power supply if it needs to be changed from what is shown in the ad.
Payment Method Accepted:
We generally accept purchase orders from government, educational, and industrial facilities in some countries. For some preapproved orders, we accept credit cards. We also accept bank wire (telegraphic) transfers. Contact us for details. We properly screen all buyers to protect against fraudulent transactions. We reserve the right to deny transactions deemed to be potentially fraudulent.
Financing Available for Select Customers:
If you are unable to afford the full purchase price, please contact us about financing details. Our financial partner offers 90 Days “Same as Cash” and deferred payments up to 6 Months.
Rental:
We have rental plans available for some companies and universities. Contact us for details.
Money Back Guarantee:
We guarantee you will be satisfied with your purchase. If in the unlikely event you are not satisfied and want to return your item, please contact us within 14 days of receiving it. We will then arrange to get you details about how to return it. We have no restocking fees but the actual cost we paid for shipping will be deducted from refund amount. We give free shipping, but for returned items, this cost will be charged to buyer.
Beware of Counterfeit Fake Parts:
Be sure of whom you are buying from as a lot of “dealers” are selling counterfeit parts, passing them off as originals. Click this link.
All Original Parts – Not Counterfeit.html
Warning: UV light and Mercury Bulbs are Dangerous! YOU MUST Read BEFORE purchasing!
Click this link.